DESCRIPTION

• Tag: C-10×His
• Host: HEK293
• Size: 100ug
• Buffer: PBS, pH7.4
• Format: Powder
• Purity: 90%
• Storage: -20℃
• Category: Cytokines&Growth Factors
• Molecule: Complement C5
• Accession: NP_001726.2
• Conjugate: Unconjugated
• Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Background: Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
• Exp Region: Gln 19 - Cys 1676
• Swiss-prot: P01031-1
• Characteristics: This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.9 kDa (β chain) and 114.4 kDa (α chain). The protein migrates as 66 kDa, 110 kDa and >180kDa under reducing (R) condition (SDS-PAGE), and >180kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Endotoxin Level: 1.0 EU per μg
• Molecular Weight: 73.9 kDa (β chain) & 114.4 kDa (α chain)
• Shipping Condition: RT
• Species Reactivity: Human
• Extra Shippinghandling Fee: Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/A48-Shipping-and-Payments.html