DESCRIPTION

• Notes: Troubleshooting Guide: Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For further questions, please email support@bpsbioscience.com
• Uniprot: A0A0G2QC41
• Shiptemp: -80°C (dry ice)
• Synonyms: LOC103689963, LOC108348065, Hdac6, Histone deacetylase 6
• Warnings: Avoid freeze/thaw cycles
• Category: Deacetylases/Assay Kit
• Background: Histone Deacetylases (HDACs) catalyze the hydrolysis of an acetyl group from acetyl-lysine present in histones. Histone acetylation/deacetylation status tightly regulates transcription. HDAC6 plays a role in stress granule formation, cell motility, and leptin sensitivity. HDAC6 interacts with heat-shock protein 90 (HSP90) and with ubiquitinated proteins and has been linked to Alzheimer's disease and tumor growth and metastasis. The use of selective inhibitors for HDAC6 offers potential benefits for neurodegenerative diseases and cancer treatment.
• References: Hey J. et al., 2023, HDAC6 score: to treat or not to treat? Nature Cancer.  4: 156-158
• Description: The rat histone deacetylase 6 (HDAC6) Assay Kit is a 96-well fluorogenic assay designed to measure rat HDAC6 enzymatic activity for screening and profiling applications. In addition, the kit includes a potent HDAC inhibitor, Trichostatin A (TSA), for use as control. The Fluorogenic Rat HDAC6 Assay Kit is based on a unique fluorogenic substrate and developer combination. A fluorescent dye is conjugated to a peptide containing acetyl-lysine. The fluorescence of the conjugated dye is quenched by the peptide. After treatment of the peptide with an HDAC, a developer solution specific for non-acetylated lysine is added to the reaction. If the acetyl group has been removed from the lysine, the developer releases the dye, allowing for fluorescence. In this assay, fluorescence directly correlates with HDAC activity.
• Methodology: Fluorogenic
• Supplied As: The kit contains enough purified HDAC6 protein, HDAC Assay Buffer, fluorogenic HDAC substrate and HDAC Developer solution for 100 reactions.
• Unspsc Code: 41105331
• Unspsc Name: Protein chemifluorescent detection reagents or kits or substrates
• Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 50006, 50057, 50687
• Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed. 
• Scientific Category: Deacetylases