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CP4-H52H3-100ug CP4-H52H3-1mg

HIV (HIV1-HXB2) Capsid protein p24, His Tag (MALS verified)

ACROBiosystems

DESCRIPTION

HIV (HIV1-HXB2) Capsid protein p24, His Tag (CP4-H52H3) is expressed from human 293 cells (HEK293). It contains AA Pro 133 - Leu 363 (Accession # P04591).

DETAILS

  • Tag: C-10×His
  • Host: HEK293
  • Size: 1mg
  • Buffer: PBS, pH7.4
  • Format: Powder
  • Purity: 90%
  • Storage: -20℃
  • Category: Others
  • Molecule: Capsid protein p24
  • Conjugate: Unconjugated
  • Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  • Background: The Gag protein from retroviruses, also known as p24, forms the inner protein layer of the nucleocapsid. It is composed of two domains, the N-terminal domain (NTD), which contributes to viral core formation, and the C-terminal domain (CTD), which is required for capsid dimerisation, Gag oligomerization and viral formation. This protein performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. ELISA tests for p24 is the most commonly used method to demonstrate virus replication both in vivo and in vitro.
  • Exp Region: Pro 133 - Leu 363
  • Swiss-prot: P04591
  • Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.5 kDa. The protein migrates as 26-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
  • Endotoxin Level: 1.0 EU per μg
  • Molecular Weight: 27.5 kDa
  • Shipping Condition: RT
  • Species Reactivity: HIV (HV1-HXB2)
  • Extra Shippinghandling Fee: Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/A48-Shipping-and-Payments.html