DESCRIPTION

• Category: ZYMO KITS
• Keywords: 11-193, 11-194, 12301-50, 12301-100, 11-142, 12311-100, 11-104, 12300-100, D4036S, D4019, QIAGEN Plasmid Mini Kit, QIAprep Spin Miniprep Kit, 12125, 27104, 27106, plasmid mini, mini kits, plasmid kits, plasmid prep, plasmid purification, endotoxin free kit, endo free, endotoxin-free, endo-free kits, Z5133
• Paragraph 1: The Zyppy™ Plasmid Miniprep Kit features a pellet-free modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify using the provided Fast-Spin column technology. Additionally, the innovative colored buffers included in the kit permit error-free visualization and identification of complete bacterial cell lysis and neutralization. The Zyppy™ Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is of the highest quality, endotoxin-free, and is well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.
• Paragraph 2: Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized. Extra Zymo-Spin Columns: Uncapped: Cat # 11-502N & 11-503N; Capped: Cat # 11-502C & 11-503C
• Bullet Point 1: The fastest, easiest miniprep available for purifying endotoxin-free transfection quality plasmid DNA
• Bullet Point 2: Purify directly from culture without the need to spin down the bacterial cells!
• Bullet Point 3: Pellet-free procedure omits conventional cell pelleting and resuspension steps
• Bullet Point 4: Colored buffers allow visualization of complete cell lysis and neutralization
• Bullet Point 5: DNA quality appropriate for cloning, sequencing, and transfection
• Bullet Point 6: Processing Time: 8 min
• Manufacturer Sku: D4019