DESCRIPTION

• Category: REAGENTS
• Keywords: cDNA Synthesis, cDNA Synthesis Kit, Reverse Trascriptase, First Strand cDNA Synthesis, mRNA, cDNA, qPCR, Real Time PCR
• Paragraph 1: UltraScript 2.0 Reverse Transcriptase is a robust and highly thermostable modified MMLV reverse transcriptase engineered for superior cDNA synthesis speed, yield and representation from a wide range of RNA sample types, including GC-rich and low abundance templates.
• Paragraph 2: The enzyme is supplied with a 5x buffer containing Mg, dNTPs, stabilizers and enhancers, optimised to generate high yield, non-biased cDNA from as little as 4pg RNA. As oligos are not included, UltraScript Reverse Transcriptase provides the convenience and flexibility for users to define their own priming strategy depending on the type of analysis needed. UltraScript Reverse Transcriptase gives exceptional performance with gene-specific primers, oligo-dT and random hexamers to produce high quality cDNA ideal for a variety of downstream applications. High quality cDNA synthesis for downstream qPCR analysis is essential for successful expression studies. Many factors affect cDNA synthesis including the reverse transcripase, buffer systems, enhancers and priming strategy. The PCRBIO cDNA synthesis mix removes the need for user optimisation of these critical factors.
• Bullet Point 1: Highly thermostable reverse transcriptase 55°C to 65°C and above
• Bullet Point 2: Superior cDNA yields from 20pg to 3.5µg RNA
• Bullet Point 3: Accurate reverse transcription of GC-rich and highly structured transcripts
• Bullet Point 4: Advanced buffer chemistry including Mg and dNTPs
• Bullet Point 5: Available as a stand-alone enzyme with buffer, a cDNA synthesis kit with premixed oligos and a cDNA synthesis kit with separate oligos
• Manufacturer Sku: PB30.33-04